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VWGDPTR001 SARS-CoV-2 whole genome sequencing of amplicons converted into sequencing ready libraries using DNA Prep Tagmentation.
VWXONTR000 Externally sequenced virus samples form Oxford Nanopore sequencing technology to be analyzed with Mutant.
MWRNXTR003 Microbial whole genome sequencing of routine samples. 3 M read pairs. Not available for all customers.
MWXNXTR003 Microbial whole genome sequencing. 3 M read pairs.
VWGNXTR001 Viral whole genome sequencing (of amplicons). 1-48 submitted samples. 1 M read pairs.
VWLNXTR001 Viral whole genome sequencing (of amplicons). >48 submitted samples. 1 M read pairs.
RMLCUSR800 RML
RMLCUSS160 RML
RMLP05R800 RML, NovaSeq SP, sequencing only, PE 2x50, 800 M r-p
RMLP15R020 RML, NovaSeq S4, sequencing only, PE 2x150, 20 M r-p
RMLP15R100 RML, NovaSeq S4, sequencing only, PE 2x150, 100 M r-p
RMLP15R200 RML, NovaSeq S4, sequencing only, PE 2x150, 200 M r-p
RMLP15R300 RML, NovaSeq S4, sequencing only, PE 2x150, 300 M r-p
RMLP15R400 RML, NovaSeq S4, sequencing only, PE 2x150, 400 M r-p
RMLP15R500 RML, NovaSeq S4, sequencing only, PE 2x150, 500 M r-p
RMLP15R750 RML, NovaSeq S4, sequencing only, PE 2x150, 750 M r-p
RMLP15S100 RML, NovaSeq S4, sequencing only, PE 2x150, 1000 M r-p
RMLP15S125 RML, NovaSeq S4, sequencing only, PE 2x150, 1250 M r-p
RMLP15S150 RML, NovaSeq S4, sequencing only, PE 2x150, 1500 M r-p
RMLP15S175 RML, NovaSeq S4, sequencing only, PE 2x150, 1750 M r-p
RMLP15S200 RML, NovaSeq S4, sequencing only, PE 2x150, 2000 M r-p
RMLP15S225 RML, NovaSeq S4, sequencing only, PE 2x150, 2250 M r-p
RMLP15S250 RML, NovaSeq S4, sequencing only, PE 2x150, 2500 M r-p
RMLP15S275 RML, NovaSeq S4, sequencing only, PE 2x150, 2750 M r-p
RMLP15S300 RML, NovaSeq S4, sequencing only, PE 2x150, 3000 M r-p
RMLP15S325 RML, NovaSeq S4, sequencing only, PE 2x150, 3250 M r-p
RMLP15S350 RML, NovaSeq S4, sequencing only, PE 2x150, 3500 M r-p
RMLP15S375 RML, NovaSeq S4, sequencing only, PE 2x150, 3750 M r-p
RMLP15S400 RML, NovaSeq S4, sequencing only, PE 2x150, 4000 M r-p
RMLP15S425 RML, NovaSeq S4, sequencing only, PE 2x150, 4250 M r-p
RMLP15S450 RML, NovaSeq S4, sequencing only, PE 2x150, 4500 M r-p
RMLP15S475 RML, NovaSeq S4, sequencing only, PE 2x150, 4750 M r-p
RMLP15S500 RML, NovaSeq S4, sequencing only, PE 2x150, 5000 M r-p
PALKTTR010 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 10 M read pairs.
PALKTTR020 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 20 M read pairs.
PALKTTR030 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 30 M read pairs.
PALKTTR040 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 40 M read pairs.
PALKTTR050 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 50 M read pairs.
PALKTTR060 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 60 M read pairs.
PALKTTR100 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 100 M read pairs. The order need to include 49 samples or more.
PANKTTR010 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 10 M read pairs.
PANKTTR020 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 20 M read pairs.
PANKTTR030 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 30 M read pairs.
PANKTTR040 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 40 M read pairs.
PANKTTR050 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 50 M read pairs.
PANKTTR060 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 60 M read pairs.
PANKTTR080 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 80 M read pairs.
PANKTTR100 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 100 M read pairs.
PANKTTR200 Panel-based sequencing using Twist Biosciences custom bait set and with Kapa Hyper Plus library preparation, 200 M read pairs.
PANWTTR020 Panel-based sequencing using Watchmaker Genomics library prep kit and Twist Biosciences custom bait set, 20 M read pairs.
EXLKTTR020 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 20 M read pairs.
EXLKTTR040 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 40 M read pairs.
EXLKTTR060 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 60 M read pairs.
EXLKTTR080 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 80 M read pairs.
EXOKTTR020 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 20 M read pairs.
EXOKTTR030 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 30 M read pairs.
EXOKTTR040 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 40 M read pairs.
EXOKTTR060 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 60 M read pairs.
EXOKTTR080 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 80 M read pairs
EXOKTTR100 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 100 M read pairs.
EXOKTTR120 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 120 M read pairs.
EXOKTTR140 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 140 M read pairs.
EXOKTTR200 Whole exome sequencing using Twist Biosciences core exome bait set and Kapa Hyper Plus library preparation, 200 M read pairs.
EXXCUSR000 Rare Disease analysis of externally sequenced whole exome data. Delivery of results in Scout.
MELLIFR030 Metagenomic sequencing
MELPCFR030 Metagenomic sequencing
METDPFR030 Metagenomic sequencing
METDPTR030 Metagenomic sequencing
METLIFR030 Metagenomic sequencing
METPCFR030 Metagenomic sequencing
METPCFR050 Metagenomic sequencing
WGLLIFC030 Whole-genome sequencing, PE 2x150, 30x coverage, Low input PCR-free library preparation. Order of 49 or more samples.
WGLLIFS120 Whole-genome sequencing, PE 2x150, 1200 M r-p. 75% of ordered quantity of data is guaranteed. Low input strategy. Order for 49 or more samples.
WGLPCFC030 Whole genome sequencing, 30X coverage. TruSeq PCR free prep. Order of 49 or more samples.
WGLPCFS120 Whole-genome sequencing, PE 2x150, 1200 M r-p. 75% of ordered quantity of data is guaranteed. TruSeq PCR-free prep. Order for 49 or more samples.
WGSDPFR020 For internal use only
WGSDPFR140 For internal use only
WGSDPFR400 Whole genome sequencing, Illumina DNA Prep PCR free.
WGSLIFC030 Whole-genome sequencing, low input strategy. PE 2x150, 30x coverage
WGSLIFR400 Whole-genome sequencing, PE 2x150, 400 M r-p. 75% of ordered quantity of data is guaranteed. Low input strategy.
WGSLIFR500 Whole-genome sequencing. This application tag should only be used when the source of DNA is muscle. PCR-free library preparation, PE 2x150, 500 M r-p. 75% of ordered quantity of data is guaranteed.
WGSLIFR800 Whole-genome sequencing, PE 2x150, 800 M r-p. 75% of ordered quantity of data is guaranteed. Low input strategy.
WGSLIFS120 Whole-genome sequencing, PE 2x150, 1200 M r-p. 75% of ordered quantity of data is guaranteed. Low input strategy.
WGSPCFC030 Whole genome sequencing, 30X coverage. TruSeq PCR free prep.
WGSPCFR010 Whole genome sequencing, 10M read pairs. TruSeq PCR free prep. Only for in-house use.
WGSPCFR400 Whole-genome sequencing, PE 2x150, 400 M r-p. 75% of ordered quantity of data is guaranteed. TruSeq PCR-free prep.
WGSPCFR500 Whole-genome sequencing. This application tag should only be used when the source of DNA is muscle. PCR-free library preparation, PE 2x150, 500 M r-p. 75% of ordered quantity of data is guaranteed.
WGSPCFR800 Whole-genome sequencing, PE 2x150, 800 M r-p. 75% of ordered quantity of data is guaranteed. TruSeq PCR-free prep.
WGSPCFS120 Whole-genome sequencing, PE 2x150, 1200 M r-p. 75% of ordered quantity of data is guaranteed. TruSeq PCR-free prep.
WGSWPFR400 Whole-genome sequencing with Watchmaker genomics PCR-free prep, PE 2x150, 400 M r-p.
WGXCUSC000 Rare Disease analysis of externally sequenced whole genome data. Delivery of results in Scout.
RNADEPR050 RNA seq, depletion of rRNA and random priming, 50 M read pairs, PE 2x150 bp.
RNADEPR100 RNA seq, depletion of rRNA, 100 M read pairs, PE 2x150 bp using NovaSeq
RNAPOAR050 RNA seq, poly-A based priming, 50 M read pairs, PE 2x150 bp using NovaSeq.
RNAPOAR075 RNA seq, poly-A based priming, 75 M read pairs, PE 2x150 bp using NovaSeq.
RNAPOAR100 RNA seq, poly-A based priming, 100 M read pairs, PE 2x150 bp using NovaSeq.
RNLPOAR050 RNA seq, poly-A based priming, 50 M read pairs, PE 2x150 bp using NovaSeq, minimum 49 samples.
RNLPOAR075 RNA seq, poly-A based priming, 75 M read pairs, PE 2x150 bp using NovaSeq, minimum 49 samples.
RNLPOAR100 RNA seq, poly-A based priming, 100 M read pairs, PE 2x150 bp using NovaSeq. For orders of 49 samples or more.
RNXCUSR000 Rare Disease analysis of externally sequenced RNA data. Delivery of results as agreed.

VWGNXTR001

Viral whole genome sequencing (of amplicons). 1-48 submitted samples. 1 M read pairs. mic

Ackreditering Nej
Svarstid 21 dagar
Min. order 1 prov
Sekvensdjup
Provmängd 50 ng
Prioritetsalternativ Nej

Detaljer

Whole-genome sequencing of viruses. Paired-end 2x150 bp using NovaSeq. The library preparation consists of tagmentation of gDNA using Nextera chemistry (Illumina) followed by amplification by annealing an unique dual index to each sample. Sequencing analysis is performed through microSALT. Sequence quality is determined by global alignment to reference, and evaluated through such metrics as insert size, duplication rate, mapping rate and coverage.

Begränsingar

Whole genome sequencing may not provide a complete coverage of the genome due to certain regions being difficult to accurately represent in sequence data. Using reference samples from National Institute of Standards and Technology in US, we estimate that >99% of the genome will be covered at 10x or more. Individual samples, especially for bacteria with a GC composition that differs from the reference genomes, may have a better or poorer performance, which should be considered in the interpretation of the results.

Senast uppdaterad: Sun Mar 27 2022